注：预变性温度和时间可以根据模板复杂度进行调整，如模板复杂度较高可以提高预变性温度并延长时间；对于长度小于200bp的扩增子，可以使用快速程序，即变性时间3秒，退火和延伸时间10秒；对于长度大于200bp的扩增子，请延长退火和延伸时间至30秒。对于高GC的扩增子，可提高退火延伸温度至65℃；循环数设置为40-45可满足绝大多数的反应；对于Tm较低的引物，可使用三步法，比如退火温度设置在55℃，延伸温度设置为72℃，信号采集设置在延伸步骤。

常见问题及解决方案：

本品仅供科研使用，不能用于人和动物医疗诊断

使用本品发表文章：

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