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HyperScript III RT SuperMix for qPCR with gDNA Remover
货 号：R202
保存条件：- 20 ℃长期储存
产品介绍：
本品为针对RT-qPCR优化的逆转录试剂盒，包含gDNA去除和逆转录两管mix，使用时只需加入RNA模板并补足水即可。高浓度的8 x gDNA remover不仅预留充足的RNA加样体积（以应对低浓度的RNA），同时可以快速去除RNA样本中残留的基因组DNA，使得qPCR的引物无需跨内含子设计。试剂盒所使用的逆转录酶是基于M-MLV定向进化而来的新一代逆转录酶，具有更低的RNase H活性、更高的热稳定性和cDNA合成效率，搭配针对qPCR优化的逆转录引物配比，可以获得在RNA各个位置逆转录效率均一的cDNA。
产品组成：
温馨提示：
·5 × RT SuperMix包含逆转录所需的全部组分，包含Randomprimers/Oligo (dT)20VN primer mix，因此不适用于基因特异性
引物进行逆转录，如有该需求推荐使用NovaBio R201。
·8 × gDNA remover、5 × RT SuperMix含有高浓度的甘油，使用前请短暂离心，避免枪头插入管底吸取试剂。
·该试剂盒的cDNA产物仅适用于qPCR实验，若需要获得全长的cDNA，推荐使用NovaBio R201。
·为保证实验成功率和准确性，请勿使用过度降解的RNA。
·配置反应液时，请使用RNase-free枪头、Microtube等。
产品使用：
1.gDNA去除体系配置及反应程序
请配置如下反应液：
混匀后42℃ 2min。
注：Total RNA建议投入量为500 ng - 1 μg。如果加入的为mRNA：10 pg - 500 ng。
2.配置逆转录反应体系
请配置如下反应液：
3.逆转录反应程序
注：1. HyperScriptTM III 在37-60℃均有较强活性，37℃即可满足绝大多数实验；若目标RNA区域有高GC部分，可提高逆转录温度至50℃。
2. 85℃ 5s 可以失活逆转录酶和gDNA remover，请勿省略该步骤。
逆转录产物可直接用于下游实验，也可置于4℃短暂放置，3-6个月内-20℃保存，长期存放请置于-80℃冰箱，小份分装避免反复冻融。
本品仅供科研使用，不能用于人和动物医疗诊断
使用本品发表文章：
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